Part:BBa_T2008:Design

c-Myc special internal domain (EQKLISEEDL)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts.

Source

This is a synthetic sequence.

References

<biblio>

1. Kolodziej P.A. Kolodziej and R.A. Young. Epitope tagging and protein surveillance. Methods Enzymol 1991; 194():508-519. doi:10.1016/0076-6879(91)94038-E
2. Terpe pmid=12536251

</biblio>